Function and Biology

Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme

Source organism: Bacillus sp. B-0618
Biochemical function: oxidoreductase activity
Biological process: oxidation-reduction process
Cellular component: cytoplasm

EC 1.5.3.1: Sarcosine oxidase

Reaction catalysed:
Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2).
Comments:
  • The flavin is both covalently and non-covalently bound in a molar ratio of 1:1.
Systematic name:
Sarcosine:oxygen oxidoreductase (demethylating)

Sequence families

Pfam Protein families (Pfam)
PF00890
Domain description: FAD binding domain
Occurring in:
  1. Monomeric sarcosine oxidase
1 copy of Pfam domain PF00890 (FAD binding domain) in Monomeric sarcosine oxidase in PDB 2gb0.

PF01266
Domain description: FAD dependent oxidoreductase
Occurring in:
  1. Monomeric sarcosine oxidase
1 copy of Pfam domain PF01266 (FAD dependent oxidoreductase) in Monomeric sarcosine oxidase in PDB 2gb0.

PF01134
Domain description: Glucose inhibited division protein A
Occurring in:
  1. Monomeric sarcosine oxidase
1 copy of Pfam domain PF01134 (Glucose inhibited division protein A) in Monomeric sarcosine oxidase in PDB 2gb0.

InterPro InterPro annotations
IPR036188
Domain description: FAD/NAD(P)-binding domain superfamily
Occurring in:
  1. Monomeric sarcosine oxidase
IPR006281
Domain description: Sarcosine oxidase, monomeric
Occurring in:
  1. Monomeric sarcosine oxidase
IPR006076
Domain description: FAD dependent oxidoreductase
Occurring in:
  1. Monomeric sarcosine oxidase

Structure domains

CATH CATH domains
3.50.50.60
Class: Alpha Beta
Architecture: 3-Layer(bba) Sandwich
Topology: FAD/NAD(P)-binding domain
Homology: FAD/NAD(P)-binding domain
Occurring in:
  1. Monomeric sarcosine oxidase
1 copy of CATH domain 3.50.50.60 (FAD/NAD(P)-binding domain) in Monomeric sarcosine oxidase in PDB 2gb0.
3.30.9.10
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: D-Amino Acid Oxidase; Chain A, domain 2
Homology: D-Amino Acid Oxidase, subunit A, domain 2
Occurring in:
  1. Monomeric sarcosine oxidase
1 copy of CATH domain 3.30.9.10 (D-Amino Acid Oxidase; Chain A, domain 2) in Monomeric sarcosine oxidase in PDB 2gb0.
SCOP SCOP annotations
51913
Class: Alpha and beta proteins (a/b)
Fold: FAD/NAD(P)-binding domain
Superfamily: FAD/NAD(P)-binding domain
Occurring in:
  1. Monomeric sarcosine oxidase
1 copy of SCOP domain 51913 (FAD-linked reductases, N-terminal domain) in Monomeric sarcosine oxidase in PDB 2gb0.
54384
Class: Alpha and beta proteins (a+b)
Fold: FAD-linked reductases, C-terminal domain
Superfamily: FAD-linked reductases, C-terminal domain
Occurring in:
  1. Monomeric sarcosine oxidase
1 copy of SCOP domain 54384 (D-aminoacid oxidase-like) in Monomeric sarcosine oxidase in PDB 2gb0.