PDBe 2g9j

Solution NMR

Complex of TM1a(1-14)Zip with TM9a(251-284): a model for the polymerization domain ("overlap region") of tropomyosin, Northeast Structural Genomics Target OR9

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tropomyosin alpha-1 chain Chains: C, D
Molecule details ›
Chains: C, D
Length: 37 amino acids
Theoretical weight: 4.18 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04692 (Residues: 251-284; Coverage: 12%)
Gene names: Alpha-tm, Tpm1, Tpma
General control protein GCN4; Tropomyosin alpha-1 chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 33 amino acids
Theoretical weight: 3.91 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P04692 (Residues: 1-14; Coverage: 5%)
  • Canonical: P03069 (Residues: 264-281; Coverage: 6%)
  • nullnull
Gene names: AAS3, ARG9, Alpha-tm, GCN4, Tpm1, Tpma, YEL009C

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 91%
Refinement method: Initail structure was calculated with Torsion Angle Dynamics and refined with simulated annealing and included a term for explicit solvent in the refinement protocol.
Chemical shifts: BMR7080  
Expression system: Escherichia coli