› 2fzu

X-ray diffraction
1.25Å resolution

Reduced enolate chromophore intermediate for GFP variant

Released:
DOI: 10.2210/pdb2fzu/pdb
PDB entry 2fzu coloured by chain, front view.
PDB entry 2fzu coloured by chain, side view.
PDB entry 2fzu coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structural evidence for an enolate intermediate in GFP fluorophore biosynthesis.
Barondeau DP, Tainer JA, Getzoff ED
J Am Chem Soc 128 3166-8 (2006)
PMID: 16522096

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.82 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand EDO 1 x EDO
1 modified residue:
Ligand C12 1 x C12

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 51.35Å b: 62.52Å c: 71.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.142 0.199
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Fluorescent proteins: maturation, photochemistry and photophysics.
Remington SJ. (2006)
4 more