PDBe 2fyn

X-ray diffraction
3.2Å resolution

Crystal Structure Analysis of the double mutant Rhodobacter Sphaeroides bc1 complex

Released:
Source organism: Rhodobacter sphaeroides
Primary publication:
Surface-modulated motion switch: capture and release of iron-sulfur protein in the cytochrome bc1 complex.
Proc. Natl. Acad. Sci. U.S.A. 103 13045-50 (2006)
PMID: 16924113

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cytochrome b Chains: A, D, G, J, M, P
Molecule details ›
Chains: A, D, G, J, M, P
Length: 445 amino acids
Theoretical weight: 50.16 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: Q02761 (Residues: 1-445; Coverage: 100%)
Gene names: fbcB, petB
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain C
Cytochrome c1 Chains: B, E, H, K, N, Q
Molecule details ›
Chains: B, E, H, K, N, Q
Length: 269 amino acids
Theoretical weight: 29.37 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: Q02760 (Residues: 23-285; Coverage: 100%)
Gene names: fbcC, petC
Sequence domains: Cytochrome C1 family
Structure domains:
Ubiquinol-cytochrome c reductase iron-sulfur subunit Chains: C, F, I, L, O, R
Molecule details ›
Chains: C, F, I, L, O, R
Length: 187 amino acids
Theoretical weight: 19.92 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: Q02762 (Residues: 1-187; Coverage: 100%)
Gene names: fbcF, petA
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 351.3Å b: 147.13Å c: 160.83Å
α: 90° β: 103.94° γ: 90°
R-values:
R R work R free
0.224 0.224 0.254
Expression system: Rhodobacter sphaeroides