PDBe 2fuz

X-ray diffraction
1.8Å resolution

UGL hexagonal crystal structure without glycine and DTT molecules

Released:
Source organism: Bacillus sp. GL1
Primary publication:
Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL1.
Biochem. Biophys. Res. Commun. 344 253-62 (2006)
PMID: 16630576

Function and Biology Details

Reaction catalysed:
Beta-D-4-deoxy-Delta(4)-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap- (1->3)-D-Glcp + H(2)O = 5-dehydro-4-deoxy-D-glucuronate + beta-D-Glcp- (1->4)-alpha-L-Rhap-(1->3)-D-Glcp. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Unsaturated glucuronyl hydrolase Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 42.91 KDa
Source organism: Bacillus sp. GL1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RC92 (Residues: 1-377; Coverage: 100%)
Gene name: ugl
Sequence domains: Glycosyl Hydrolase Family 88
Structure domains: Glycosyltransferase

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P6522
Unit cell:
a: 102.818Å b: 102.818Å c: 223.156Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.167 0.188
Expression system: Escherichia coli BL21(DE3)