PDBe 2fum

X-ray diffraction
2.89Å resolution

Catalytic domain of protein kinase PknB from Mycobacterium tuberculosis in complex with mitoxantrone

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PknB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 299 amino acids
Theoretical weight: 32.46 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WI81 (Residues: 1-279; Coverage: 45%)
Gene names: MTCY10H4.14c, Rv0014c, pknB
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P43212
Unit cell:
a: 116.863Å b: 116.863Å c: 260.347Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.278
Expression system: Escherichia coli BL21(DE3)