› 2frz

X-ray diffraction
2.1Å resolution

Crystal Structure of Cytochrome P450cam mutant (F87W/Y96F/V247L/C334A)

Released:
DOI: 10.2210/pdb2frz/pdb
PDB entry 2frz coloured by chain, front view.
PDB entry 2frz coloured by chain, side view.
PDB entry 2frz coloured by chain, top view.
Source organism: Pseudomonas putida
Entry authors: Rao Z, Wong LL, Xu F, Bell SG

Function and Biology Details

Reaction catalysed: 
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 414 amino acids
Theoretical weight: 46.59 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00183 (Residues: 2-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
1 bound ligand:
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 66.816Å b: 62.114Å c: 94.94Å
α: 90° β: 90.46° γ: 90°
R-values:
R R work R free
0.21 0.19 0.243
Expression system: Escherichia coli BL21(DE3)

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