PDBe 2frv

X-ray diffraction
2.54Å resolution

CRYSTAL STRUCTURE OF THE OXIDIZED FORM OF NI-FE HYDROGENASE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Periplasmic [NiFe] hydrogenase small subunit Chains: A, C, E, G, I, S
Molecule details ›
Chains: A, C, E, G, I, S
Length: 264 amino acids
Theoretical weight: 28.35 KDa
Source organism: Desulfovibrio gigas
UniProt:
  • Canonical: P12943 (Residues: 25-288; Coverage: 100%)
Gene name: hydA
Sequence domains:
Structure domains:
Periplasmic [NiFe] hydrogenase large subunit Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 536 amino acids
Theoretical weight: 59.57 KDa
Source organism: Desulfovibrio gigas
UniProt:
  • Canonical: P12944 (Residues: 1-536; Coverage: 97%)
Gene name: hydB
Sequence domains: Nickel-dependent hydrogenase
Structure domains: Cytochrome-c3 Hydrogenase, chain B

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID2
Spacegroup: P1
Unit cell:
a: 112.78Å b: 113.16Å c: 133.91Å
α: 90.03° β: 90.02° γ: 119.99°
R-values:
R R work R free
0.225 0.201 0.219