PDBe 2fok

X-ray diffraction
2.3Å resolution

STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI

Released:
Source organism: Planomicrobium okeanokoites
Primary publication:
Structure of FokI has implications for DNA cleavage.
Proc. Natl. Acad. Sci. U.S.A. 95 10564-9 (1998)
PMID: 9724743

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type-2 restriction enzyme FokI Chains: A, B
Molecule details ›
Chains: A, B
Length: 579 amino acids
Theoretical weight: 65.83 KDa
Source organism: Planomicrobium okeanokoites
Expression system: Escherichia coli
UniProt:
  • Canonical: P14870 (Residues: 5-583; Coverage: 99%)
Gene names: fokIR, rfoKI
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 54.01Å b: 137.17Å c: 188.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.306
Expression system: Escherichia coli