PDBe 2fo5

X-ray diffraction
2.2Å resolution

Crystal structure of recombinant barley cysteine endoprotease B isoform 2 (EP-B2) in complex with leupeptin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cysteine proteinase EP-B 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 262 amino acids
Theoretical weight: 27.76 KDa
Source organism: Hordeum vulgare
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P25250 (Residues: 131-373; Coverage: 70%)
Gene name: EPB2
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases
ACE-LEU-LEU-argininal (leupeptin) Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 4 amino acids
Theoretical weight: 430 Da
Source organism: Streptomyces roseus
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 44.37Å b: 51.73Å c: 115.655Å
α: 86.32° β: 86.46° γ: 79.63°
R-values:
R R work R free
0.201 0.201 0.233
Expression systems:
  • Escherichia coli BL21
  • Not provided