Function and Biology

Ala177Val mutant of E. coli Methylenetetrahydrofolate Reductase complex with LY309887

Source organism: Escherichia coli

EC 1.5.1.54: Methylenetetrahydrofolate reductase (NADH)

Reaction catalysed:
5-methyltetrahydrofolate + NAD(+) = 5,10-methylenetetrahydrofolate + NADH
Systematic name:
5-methyltetrahydrofolate:NAD(+) oxidoreductase
Alternative Name(s):
  • 5,10-CH(2)-H(4)folate reductase
  • 5,10-methylenetetrahydrofolate reductase
  • 5,10-methylenetetrahydrofolate reductase (FADH(2))
  • 5,10-methylenetetrahydrofolic acid reductase
  • 5,10-methylenetetrahydropteroylglutamate reductase
  • 5-methyltetrahydrofolate:(acceptor) oxidoreductase
  • Methylenetetrahydrofolate (reduced riboflavin adenine dinucleotide) reductase
  • Methylenetetrahydrofolate reductase
  • Methylenetetrahydrofolic acid reductase
  • N(5),10-methylenetetrahydrofolate reductase
  • N(5),N(10)-methylenetetrahydrofolate reductase

Sequence family

Pfam Protein family (Pfam)
PF02219
Domain description: Methylenetetrahydrofolate reductase
Occurring in:
  1. 5,10-methylenetetrahydrofolate reductase
The deposited structure of PDB entry 2fmn contains 3 copies of Pfam domain PF02219 (Methylenetetrahydrofolate reductase) in 5,10-methylenetetrahydrofolate reductase. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR003171
Domain description: Methylenetetrahydrofolate reductase-like
Occurring in:
  1. 5,10-methylenetetrahydrofolate reductase
IPR004620
Domain description: 5,10-methylenetetrahydrofolate reductase
Occurring in:
  1. 5,10-methylenetetrahydrofolate reductase
IPR029041
Domain description: FAD-linked oxidoreductase-like
Occurring in:
  1. 5,10-methylenetetrahydrofolate reductase

Structure domain

CATH CATH domain
3.20.20.220
Class: Alpha Beta
Architecture: Alpha-Beta Barrel
Topology: TIM Barrel
Homology: TIM Barrel
Occurring in:
  1. 5,10-methylenetetrahydrofolate reductase
The deposited structure of PDB entry 2fmn contains 3 copies of CATH domain 3.20.20.220 (TIM Barrel) in 5,10-methylenetetrahydrofolate reductase. Showing 1 copy in chain A.