PDBe 2fka

X-ray diffraction
2Å resolution

Crystal structure of Mg(2+) and BeF(3)(-)-bound CheY in complex with CheZ(200-214) solved from a F432 crystal grown in CAPS (pH 10.5)

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
homo 12-mer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chemotaxis protein CheY Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.14 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A2D5 (Residues: 1-129; Coverage: 100%)
Gene names: STM1916, cheY
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold
Protein phosphatase CheZ Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.62 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Not provided
UniProt:
  • Canonical: P07800 (Residues: 200-214; Coverage: 7%)
Gene names: STM1915, cheZ

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: F432
Unit cell:
a: 198.73Å b: 198.73Å c: 198.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.224
Expression systems:
  • Escherichia coli
  • Not provided