› 2ff7

X-ray diffraction
1.6Å resolution

The ABC-ATPase of the ABC-transporter HlyB in the ADP bound state

Released:
DOI: 10.2210/pdb2ff7/pdb
PDB entry 2ff7 coloured by chain, front view.
PDB entry 2ff7 coloured by chain, side view.
PDB entry 2ff7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A structural analysis of asymmetry required for catalytic activity of an ABC-ATPase domain dimer.
Zaitseva J, Oswald C, Jumpertz T, Jenewein S, Wiedenmann A, Holland IB, Schmitt L
EMBO J 25 3432-43 (2006)
PMID: 16858415

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-hemolysin translocation ATP-binding protein HlyB Chain: A
Molecule details ›
Chain: A
Length: 247 amino acids
Theoretical weight: 27.75 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08716 (Residues: 467-707; Coverage: 34%)
Gene name: hlyB
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: C2
Unit cell:
a: 180.37Å b: 34.837Å c: 37.82Å
α: 90° β: 98.41° γ: 90°
R-values:
R R work R free
0.194 0.192 0.231
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Structure, function, and evolution of bacterial ATP-binding cassette systems.
Davidson et al. (2008)
19 more

7 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
6 more