PDBe 2ff0

Solution NMR

Solution Structure of Steroidogenic Factor 1 DNA Binding Domain Bound to its Target Sequence in the Inhibin alpha-subunit Promoter

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Steroidogenic factor 1 Chain: A
Molecule details ›
Chain: A
Length: 102 amino acids
Theoretical weight: 11.89 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33242 (Residues: 10-111; Coverage: 22%)
Gene names: Ftzf1, Nr5a1
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
CTGTGGCCCTGAGCC Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.56 KDa
Source organism: Synthetic construct
Expression system: Not provided
GGCTCAGGGCCACAG Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.62 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: Simulated annealing and cartesian molecular dynamics
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided