PDBe 2fek

Solution NMR

Structure of a protein tyrosine phosphatase

Released:

Function and Biology Details

Reaction catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate. 
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Low molecular weight protein-tyrosine-phosphatase Wzb Chain: A
Molecule details ›
Chain: A
Length: 167 amino acids
Theoretical weight: 18.9 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AAB2 (Residues: 1-147; Coverage: 100%)
Gene names: JW2046, b2061, wzb
Sequence domains: Low molecular weight phosphotyrosine protein phosphatase
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 88%
Refinement method: 200 structures were generated in CYANA. The 100 structures with lowest energy were further refined in AMBER7. The 20 lowest energy structures were finally selected.
Chemical shifts: BMR6934  
Expression system: Escherichia coli BL21(DE3)