PDBe 2fci

Solution NMR

Structural basis for the requirement of two phosphotyrosines in signaling mediated by Syk tyrosine kinase

Released:

Function and Biology Details

Reaction catalysed:
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 12.28 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08487 (Residues: 663-759; Coverage: 8%)
Gene name: PLCG1
Sequence domains: SH2 domain
Tyrosine-protein kinase Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.7 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: Q32PK0 (Residues: 314-326; Coverage: 2%)
Gene name: SYK

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided