PDBe 2fb9

X-ray diffraction
1.9Å resolution

Crystal structure of the Apo form of D-alanine: D-alanine ligase (Ddl) from Thermus caldophilus: a basis for the substrate-induced conformational changes

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-alanine--D-alanine ligase Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 35.11 KDa
Source organism: Thermus caldophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3T920 (Residues: 1-319; Coverage: 100%)
Gene name: ddl
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: C2221
Unit cell:
a: 115.216Å b: 132.744Å c: 59.324Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.228 0.268
Expression system: Escherichia coli