PDBe 2f9z

X-ray diffraction
2.4Å resolution

Complex between the chemotaxis deamidase CheD and the chemotaxis phosphatase CheC from Thermotoga maritima

Released:

Function and Biology Details

Reactions catalysed:
Protein L-glutamine + H(2)O = protein L-glutamate + NH(3). 
Protein L-glutamate O(5)-methyl ester + H(2)O = protein L-glutamate + methanol. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CheY-P phosphatase CheC Chains: A, B
Molecule details ›
Chains: A, B
Length: 205 amino acids
Theoretical weight: 22.57 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X006 (Residues: 1-205; Coverage: 100%)
Gene names: TM_0904, cheC
Sequence domains: CheC-like family
Structure domains: CheC-like
Chemoreceptor glutamine deamidase CheD Chains: C, D
Molecule details ›
Chains: C, D
Length: 159 amino acids
Theoretical weight: 16.89 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X005 (Residues: 1-157; Coverage: 100%)
Gene names: TM_0903, cheD
Sequence domains: CheD chemotactic sensory transduction

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P32
Unit cell:
a: 66.08Å b: 66.08Å c: 161.76Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.211 0.275
Expression system: Escherichia coli BL21(DE3)