PDBe 2f9r

X-ray diffraction
1.85Å resolution

Crystal structure of the inactive state of the Smase I, a sphingomyelinase D from Loxosceles laeta venom

Released:

Function and Biology Details

Reaction catalysed:
A phosphatidylcholine + H(2)O = choline + a phosphatidate. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase D LlSicTox-alphaIII1i Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 285 amino acids
Theoretical weight: 32.08 KDa
Source organism: Loxosceles laeta
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8I914 (Residues: 27-311; Coverage: 98%)
Structure domains: Phosphatidylinositol (PI) phosphodiesterase

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: P65
Unit cell:
a: 140.587Å b: 140.587Å c: 113.608Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.187 0.234
Expression system: Escherichia coli BL21