PDBe 2f8t

X-ray diffraction
3.1Å resolution

Crystal structure of Aa-Ago with externally-bound siRNA

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
monomeric
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Argonaute protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 706 amino acids
Theoretical weight: 83.26 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67434 (Residues: 1-706; Coverage: 100%)
Gene name: aq_1447
Sequence domains: Piwi domain
Structure domains:
26-MER Chains: C, D
Molecule details ›
Chains: C, D
Length: 26 nucleotides
Theoretical weight: 8.28 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 81.2Å b: 118.077Å c: 98.498Å
α: 90° β: 99.57° γ: 90°
R-values:
R R work R free
0.25 0.204 0.297
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided