PDBe 2f8s

X-ray diffraction
3Å resolution

Crystal structure of Aa-Ago with externally-bound siRNA

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
monomeric
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Argonaute protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 706 amino acids
Theoretical weight: 83.26 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67434 (Residues: 1-706; Coverage: 100%)
Gene name: aq_1447
Sequence domains: Piwi domain
Structure domains:
5'-R(P*AP*GP*AP*CP*AP*GP*CP*AP*UP*AP*UP*AP*UP*GP*CP*UP*GP*UP*CP*UP*UP*U)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 22 nucleotides
Theoretical weight: 6.98 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 79.697Å b: 117.516Å c: 98.62Å
α: 90° β: 99.12° γ: 90°
R-values:
R R work R free
0.25 0.212 0.307
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided