PDBe 2f5s

X-ray diffraction
2.35Å resolution

Catalytically inactive (E3Q) MutM crosslinked to oxoG:C containing DNA CC1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Formamidopyrimidine-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 30.71 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P84131 (Residues: 1-274; Coverage: 100%)
Gene names: fpg, mutM
Sequence domains:
Structure domains:
5'-D(*AP*GP*GP*TP*AP*GP*AP*CP*TP*CP*GP*GP*AP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.95 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*TP*GP*C*GP*TP*CP*CP*(8OG)P*AP*GP*TP*CP*TP*AP*CP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.87 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 45.395Å b: 93.366Å c: 103.705Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.261
Expression systems:
  • Escherichia coli
  • Not provided