› 2f4q

X-ray diffraction
1.75Å resolution

Crystal Structure of Deinococcus radiodurans topoisomerase IB

Released:
DOI: 10.2210/pdb2f4q/pdb
PDB entry 2f4q coloured by chain, front view.
PDB entry 2f4q coloured by chain, side view.
PDB entry 2f4q coloured by chain, top view.
Source organism: Deinococcus radiodurans
Primary publication:
Crystal structure of a bacterial type IB DNA topoisomerase reveals a preassembled active site in the absence of DNA.
Patel A, Shuman S, Mondragón A
J Biol Chem 281 6030-7 (2006)
PMID: 16368685

Function and Biology Details

Reaction catalysed: 
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193317 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase Chain: A
Molecule details ›
Chain: A
Length: 346 amino acids
Theoretical weight: 39.31 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9RWH8 (Residues: 1-346; Coverage: 100%)
Gene name: DR_0690
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P21
Unit cell:
a: 38.094Å b: 64.966Å c: 76.622Å
α: 90° β: 91.78° γ: 90°
R-values:
R R work R free
0.199 0.197 0.231
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

DNA topoisomerases: harnessing and constraining energy to govern chromosome topology.
Schoeffler et al. (2008)
2 more

4 mentions without citation

Phylogenomics of DNA topoisomerases: their origin and putative roles in the emergence of modern organisms.
Forterre et al. (2009)
3 more