› 2f3r

X-ray diffraction
2.5Å resolution

Crystal Structure Of E.coli Guanylate Kinase In Complex With Ap5G

Released:
DOI: 10.2210/pdb2f3r/pdb
PDB entry 2f3r coloured by chain, front view.
PDB entry 2f3r coloured by chain, side view.
PDB entry 2f3r coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of GMP kinase in complex with ganciclovir monophosphate and Ap5G.
Hible G, Daalova P, Gilles AM, Cherfils J
Biochimie 88 1157-64 (2006)
PMID: 16690197

Function and Biology Details

Reaction catalysed: 
ATP + GMP = ADP + GDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158041 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 23.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60546 (Residues: 1-207; Coverage: 100%)
Gene names: JW3623, b3648, gmk, spoR
Sequence domains: Guanylate kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand G5P 2 x G5P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P63
Unit cell:
a: 67.527Å b: 67.527Å c: 149.703Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.211 0.247
Expression system: Escherichia coli

Quick links

Citations

11 citation in other articles

FBA reveals guanylate kinase as a potential target for antiviral therapies against SARS-CoV-2.
Renz et al. (2020)
10 more

1 mention without citation

The ESEV PDZ-binding motif of the avian influenza A virus NS1 protein protects infected cells from apoptosis by directly targeting Scribble.
Liu et al. (2010)