PDBe 2f2a

X-ray diffraction
2.3Å resolution

Structure of tRNA-Dependent Amidotransferase GatCAB complexed with Gln

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Glutamyl-tRNA(Gln) amidotransferase subunit A Chain: A
Molecule details ›
Chain: A
Length: 485 amino acids
Theoretical weight: 52.86 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63488 (Residues: 1-485; Coverage: 100%)
Gene names: SAV1900, gatA
Sequence domains: Amidase
Structure domains: Amidase signature (AS) domain
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B Chain: B
Molecule details ›
Chain: B
Length: 483 amino acids
Theoretical weight: 54.79 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P64201 (Residues: 1-475; Coverage: 100%)
Gene names: SAV1899, gatB
Sequence domains:
Structure domains: DNA polymerase; domain 1
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C Chain: C
Molecule details ›
Chain: C
Length: 100 amino acids
Theoretical weight: 11.28 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P68807 (Residues: 1-100; Coverage: 100%)
Gene names: SAV1901, gatC
Sequence domains: Glu-tRNAGln amidotransferase C subunit
Structure domains: Histone, subunit A

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 77.187Å b: 88.222Å c: 183.401Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.252
Expression system: Escherichia coli