PDBe 2ez6

X-ray diffraction
2.05Å resolution

Crystal structure of Aquifex aeolicus RNase III (D44N) complexed with product of double-stranded RNA processing

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 26.15 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67082 (Residues: 1-221; Coverage: 100%)
Gene names: aq_946, rnc
Sequence domains:
Structure domains:
28-MER Chains: C, D
Molecule details ›
Chains: C, D
Length: 28 nucleotides
Theoretical weight: 8.98 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 63.839Å b: 50.976Å c: 113.746Å
α: 90° β: 104.47° γ: 90°
R-values:
R R work R free
0.215 0.205 0.258
Expression systems:
  • Escherichia coli
  • Not provided