PDBe 2eua

X-ray diffraction
2.5Å resolution

Structure and Mechanism of MenF, the Menaquinone-Specific Isochorismate Synthase from Escherichia Coli

Released:

Function and Biology Details

Reaction catalysed:
Chorismate = isochorismate. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isochorismate synthase MenF Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 48.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P38051 (Residues: 1-431; Coverage: 100%)
Gene names: JW2260, b2265, menF, yfbA
Sequence domains: chorismate binding enzyme
Structure domains: Anthranilate synthase

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P43212
Unit cell:
a: 146.408Å b: 146.408Å c: 125.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.229 0.276
Expression system: Escherichia coli