› 2epn

X-ray diffraction
1.61Å resolution

N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii

Released:
DOI: 10.2210/pdb2epn/pdb
PDB entry 2epn coloured by chain, front view.
PDB entry 2epn coloured by chain, side view.
PDB entry 2epn coloured by chain, top view.
Source organism: Streptococcus gordonii
Primary publication:
Structure of N-acetyl-beta-D-glucosaminidase (GcnA) from the endocarditis pathogen Streptococcus gordonii and its complex with the mechanism-based inhibitor NAG-thiazoline.
Langley DB, Harty DW, Jacques NA, Hunter N, Guss JM, Collyer CA
J Mol Biol 377 104-16 (2008)
PMID: 18237743

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180336 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyl-beta-D-glucosaminidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 627 amino acids
Theoretical weight: 72.33 KDa
Source organism: Streptococcus gordonii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6ST21 (Residues: 1-627; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NGT 2 x NGT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 107.574Å b: 112.332Å c: 103.127Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.202
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Glycoside hydrolases: catalytic base/nucleophile diversity.
Vuong et al. (2010)
20 other articles

2 mentions without citation

A Second β-Hexosaminidase Encoded in the Streptococcus pneumoniae Genome Provides an Expanded Biochemical Ability to Degrade Host Glycans.
Robb et al. (2015)
1 more