PDBe 2eij

X-ray diffraction
1.9Å resolution

Bovine heart cytochrome C oxidase in the fully reduced state

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 26-mer (preferred)
Entry contents:
13 distinct polypeptide molecules
Macromolecules (13 distinct):
Cytochrome c oxidase subunit 1 Chains: A, N
Molecule details ›
Chains: A, N
Length: 514 amino acids
Theoretical weight: 57.09 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00396 (Residues: 1-514; Coverage: 100%)
Gene names: COI, COXI, MT-CO1, MTCO1
Sequence domains: Cytochrome C and Quinol oxidase polypeptide I
Structure domains: Cytochrome C Oxidase, chain A
Cytochrome c oxidase subunit 2 Chains: B, O
Molecule details ›
Chains: B, O
Length: 227 amino acids
Theoretical weight: 26.07 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P68530 (Residues: 1-227; Coverage: 100%)
Gene names: COII, COXII, MT-CO2, MTCO2
Sequence domains:
Structure domains:
Cytochrome c oxidase subunit 3 Chains: C, P
Molecule details ›
Chains: C, P
Length: 261 amino acids
Theoretical weight: 29.96 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00415 (Residues: 1-261; Coverage: 100%)
Gene names: COIII, COXIII, MT-CO3, MTCO3
Sequence domains: Cytochrome c oxidase subunit III
Structure domains:
Cytochrome c oxidase subunit 5A, mitochondrial Chains: E, R
Molecule details ›
Chains: E, R
Length: 109 amino acids
Theoretical weight: 12.45 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00426 (Residues: 44-152; Coverage: 72%)
Gene name: COX5A
Sequence domains: Cytochrome c oxidase subunit Va
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Cytochrome c oxidase subunit 5B, mitochondrial Chains: F, S
Molecule details ›
Chains: F, S
Length: 98 amino acids
Theoretical weight: 10.68 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00428 (Residues: 32-129; Coverage: 76%)
Gene name: COX5B
Structure domains: Cytochrome C Oxidase, chain F
Cytochrome c oxidase subunit 6C Chains: I, V
Molecule details ›
Chains: I, V
Length: 73 amino acids
Theoretical weight: 8.54 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P04038 (Residues: 2-74; Coverage: 99%)
Gene name: COX6C
Sequence domains: Cytochrome c oxidase subunit VIc
Structure domains: Cytochrome C Oxidase, chain I
Cytochrome c oxidase subunit 7B, mitochondrial Chains: K, X
Molecule details ›
Chains: K, X
Length: 56 amino acids
Theoretical weight: 6.37 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13183 (Residues: 25-80; Coverage: 70%)
Gene name: COX7B
Structure domains: Cytochrome C Oxidase, chain K
Cytochrome c oxidase subunit 7C, mitochondrial Chains: L, Y
Molecule details ›
Chains: L, Y
Length: 47 amino acids
Theoretical weight: 5.45 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00430 (Residues: 17-63; Coverage: 75%)
Gene names: COX7C, COX7CP1
Structure domains: Cytochrome C Oxidase, chain L
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial Chains: D, Q
Molecule details ›
Chains: D, Q
Length: 147 amino acids
Theoretical weight: 17.18 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00423 (Residues: 23-169; Coverage: 87%)
Gene names: COX4, COX4I1
Sequence domains: Cytochrome c oxidase subunit IV
Structure domains: Cytochrome C Oxidase, chain D
Cytochrome c oxidase subunit 6A2, mitochondrial Chains: G, T
Molecule details ›
Chains: G, T
Length: 85 amino acids
Theoretical weight: 9.63 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P07471 (Residues: 13-97; Coverage: 88%)
Gene names: COX6A, COX6A2
Structure domains: Cytochrome C Oxidase, chain G
Cytochrome c oxidase subunit 6B1 Chains: H, U
Molecule details ›
Chains: H, U
Length: 85 amino acids
Theoretical weight: 10.04 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00429 (Residues: 2-86; Coverage: 99%)
Gene names: COX6B, COX6B1
Sequence domains: Cytochrome oxidase c subunit VIb
Structure domains: Cytochrome C oxidase subunit h
Cytochrome c oxidase subunit 8B, mitochondrial Chains: M, Z
Molecule details ›
Chains: M, Z
Length: 46 amino acids
Theoretical weight: 4.97 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P10175 (Residues: 25-70; Coverage: 66%)
Gene names: COX8B, COX8H
Sequence domains: Cytochrome oxidase c subunit VIII
Structure domains: Cytochrome C Oxidase, chain M
Cytochrome c oxidase subunit 7A1, mitochondrial Chains: J, W
Molecule details ›
Chains: J, W
Length: 59 amino acids
Theoretical weight: 6.68 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P07470 (Residues: 22-80; Coverage: 74%)
Gene names: COX7A, COX7A1, COX7AH
Sequence domains: Cytochrome c oxidase subunit VII
Structure domains: Cytochrome C Oxidase, chain J

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 183.06Å b: 206.584Å c: 178.298Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.234