› 2e7f

X-ray diffraction
2.2Å resolution

5-methyltetrahydrofolate corrinoid/iron sulfur protein methyltransferase complexed with methyltetrahydrofolate to 2.2 Angsrom resolution

Released:
DOI: 10.2210/pdb2e7f/pdb
PDB entry 2e7f coloured by chain, front view.
PDB entry 2e7f coloured by chain, side view.
PDB entry 2e7f coloured by chain, top view.
Source organism: Moorella thermoacetica
Primary publication:
Structural and kinetic evidence for an extended hydrogen-bonding network in catalysis of methyl group transfer. Role of an active site asparagine residue in activation of methyl transfer by methyltransferases.
Doukov TI, Hemmi H, Drennan CL, Ragsdale SW
J Biol Chem 282 6609-6618 (2007)
PMID: 17172470

Function and Biology Details

Reaction catalysed: 
A [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate = a [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175114 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-methyltetrahydrofolate:corrinoid/iron-sulfur protein co-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 28.64 KDa
Source organism: Moorella thermoacetica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46389 (Residues: 1-262; Coverage: 100%)
Gene name: acsE
Sequence domains: Pterin binding enzyme
Structure domains: Dihydropteroate synthase-like

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand C2F 2 x C2F
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 50.159Å b: 78.547Å c: 135.617Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.172 0.23
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Acetogenesis and the Wood-Ljungdahl pathway of CO(2) fixation.
Ragsdale et al. (2008)
7 more

2 mentions without citation

Visualizing molecular juggling within a B12-dependent methyltransferase complex.
Kung et al. (2012)
1 more