PDBe 2e33

X-ray diffraction
2.7Å resolution

Structural basis for selection of glycosylated substrate by SCFFbs1 ubiquitin ligase

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
F-box only protein 2 Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 22.59 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q80UW2 (Residues: 105-297; Coverage: 65%)
Gene names: Fbs1, Fbx2, Fbxo2
Sequence domains: F-box associated region
Structure domains: Galactose-binding domain-like
Ribonuclease pancreatic Chain: B
Molecule details ›
Chain: B
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: P-30 Protein

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P432
Unit cell:
a: 148.314Å b: 148.314Å c: 148.314Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.216 0.288
Expression system: Escherichia coli