› 2e2x

X-ray diffraction
2.5Å resolution

Sec14 Homology Module of Neurofibromin in complex with phosphatitylethanolamine

Released:
DOI: 10.2210/pdb2e2x/pdb
PDB entry 2e2x coloured by chain, front view.
PDB entry 2e2x coloured by chain, side view.
PDB entry 2e2x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The sec14 homology module of neurofibromin binds cellular glycerophospholipids: mass spectrometry and structure of a lipid complex.
Welti S, Fraterman S, D'Angelo I, Wilm M, Scheffzek K
J Mol Biol 366 551-62 (2007)
PMID: 17187824

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149214 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurofibromin Chains: A, B
Molecule details ›
Chains: A, B
Length: 277 amino acids
Theoretical weight: 31.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21359 (Residues: 1566-1837; Coverage: 10%)
Gene name: NF1
Sequence domains: Divergent CRAL/TRIO domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PEV 2 x PEV
Ligand POP 2 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41212
Unit cell:
a: 110.1Å b: 110.1Å c: 121.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.254 0.283
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
18 more

4 mentions without citation

The Sec14 superfamily and mechanisms for crosstalk between lipid metabolism and lipid signaling.
Bankaitis et al. (2010)
3 more