› 2e1b

X-ray diffraction
2.7Å resolution

Crystal structure of the AlaX-M trans-editing enzyme from Pyrococcus horikoshii

Released:
DOI: 10.2210/pdb2e1b/pdb
PDB entry 2e1b coloured by chain, front view.
PDB entry 2e1b coloured by chain, side view.
PDB entry 2e1b coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
Structure of the AlaX-M trans-editing enzyme from Pyrococcus horikoshii.
Fukunaga R, Yokoyama S
Acta Crystallogr D Biol Crystallogr 63 390-400 (2007)
PMID: 17327676

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanyl-tRNA editing protein AlaX-M Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 25.34 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O57848 (Residues: 1-216; Coverage: 100%)
Gene names: PH0108, alaXM
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P62
Unit cell:
a: 80.223Å b: 80.223Å c: 72.269Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.227 0.313
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Aminoacyl-tRNA synthetase complexes: molecular multitasking revealed.
Hausmann et al. (2008)
3 more

4 mentions without citation

Unique protein architecture of alanyl-tRNA synthetase for aminoacylation, editing, and dimerization.
Naganuma et al. (2009)
3 more