PDBe 2dzn

X-ray diffraction
2.2Å resolution

Crystal structure analysis of yeast Nas6p complexed with the proteasome subunit, rpt3

Released:

Function and Biology Details

Biochemical function:
Cellular component:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
26S protease regulatory subunit 6B homolog Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P33298 (Residues: 348-428; Coverage: 19%)
Gene names: D9509.14, RPT3, YDR394W, YNT1, YTA2
Structure domains: Helicase, Ruva Protein; domain 3
Probable 26S proteasome regulatory subunit p28 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 228 amino acids
Theoretical weight: 25.65 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P50086 (Residues: 1-228; Coverage: 100%)
Gene names: G8564, NAS6, YGR232W
Sequence domains: Ankyrin repeat
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 60.376Å b: 100.22Å c: 72.203Å
α: 90° β: 94.7° γ: 90°
R-values:
R R work R free
0.197 0.197 0.265
Expression system: Escherichia coli