› 2dut

X-ray diffraction
3Å resolution

Crystal structure of a M-loop deletion variant of MENT in the native conformation

Released:
DOI: 10.2210/pdb2dut/pdb
PDB entry 2dut coloured by chain, front view.
PDB entry 2dut coloured by chain, side view.
PDB entry 2dut coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
X-ray crystal structure of MENT: evidence for functional loop-sheet polymers in chromatin condensation.
McGowan S, Buckle AM, Irving JA, Ong PC, Bashtannyk-Puhalovich TA, Kan WT, Henderson KN, Bulynko YA, Popova EY, Smith AI, Bottomley SP, Rossjohn J, Grigoryev SA, Pike RN, Whisstock JC
EMBO J 25 3144-55 (2006)
PMID: 16810322

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130725 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heterochromatin-associated protein MENT Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 423 amino acids
Theoretical weight: 48.86 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: O73790 (Residues: 1-410; Coverage: 93%)
Gene names: MENT, SERPINB10
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P32
Unit cell:
a: 182.559Å b: 182.559Å c: 96.723Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.278 0.278 0.293
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Conformational pathology of the serpins: themes, variations, and therapeutic strategies.
Gooptu et al. (2009)
9 more

1 mention without citation

1.45 Å resolution structure of SRPN18 from the malaria vector Anopheles gambiae.
Meekins et al. (2016)