› 2dtx

X-ray diffraction
1.6Å resolution

Structure of Thermoplasma acidophilum aldohexose dehydrogenase (AldT) in complex with D-mannose

Released:
DOI: 10.2210/pdb2dtx/pdb
PDB entry 2dtx coloured by chain, front view.
PDB entry 2dtx coloured by chain, side view.
PDB entry 2dtx coloured by chain, top view.
Source organism: Thermoplasma acidophilum
Primary publication:
Structural insights into unique substrate selectivity of Thermoplasma acidophilum D-aldohexose dehydrogenase.
Yasutake Y, Nishiya Y, Tamura N, Tamura T
J Mol Biol 367 1034-46 (2007)
PMID: 17300803

Function and Biology Details

Reaction catalysed: 
D-glucose + NAD(+) = D-glucono-1,5-lactone + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190872 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose 1-dehydrogenase related protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 29.03 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HK51 (Residues: 2-255; Coverage: 100%)
Gene name: Ta0754
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand BMA 2 x BMA
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P3221
Unit cell:
a: 81.879Å b: 81.879Å c: 138.279Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.198 0.221
Expression system: Escherichia coli

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Citations

10 citation in other articles

Protein structure modeling indicates hexahistidine-tag interference with enzyme activity.
Freydank et al. (2008)
9 more