PDBe 2dsr

X-ray diffraction
2.1Å resolution

Structural Basis for the Inhibition of Insulin-like Growth Factors by IGF Binding Proteins

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Insulin-like growth factor I Chain: I
Molecule details ›
Chain: I
Length: 70 amino acids
Theoretical weight: 7.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05019 (Residues: 49-118; Coverage: 40%)
Gene names: IBP1, IGF1
Sequence domains: Insulin/IGF/Relaxin family
Structure domains: Insulin-like, subunit E
Insulin-like growth factor-binding protein 4 Chain: G
Molecule details ›
Chain: G
Length: 82 amino acids
Theoretical weight: 9.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22692 (Residues: 172-253; Coverage: 35%)
Gene names: IBP4, IGFBP4
Sequence domains: Thyroglobulin type-1 repeat
Insulin-like growth factor-binding protein 4 Chain: B
Molecule details ›
Chain: B
Length: 80 amino acids
Theoretical weight: 8.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22692 (Residues: 24-103; Coverage: 34%)
Gene names: IBP4, IGFBP4
Sequence domains: Insulin-like growth factor binding protein

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 74.4Å b: 50.25Å c: 64.3Å
α: 90° β: 115.3° γ: 90°
R-values:
R R work R free
0.202 0.199 0.256
Expression system: Escherichia coli BL21(DE3)