PDBe 2dsq

X-ray diffraction
2.8Å resolution

Structural Basis for the Inhibition of Insulin-like Growth Factors by IGF Binding Proteins

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
IGF1 protein Chains: C, I
Molecule details ›
Chains: C, I
Length: 70 amino acids
Theoretical weight: 7.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NP10 (Residues: 26-95; Coverage: 67%)
Gene name: IGF1
Sequence domains: Insulin/IGF/Relaxin family
Structure domains: Insulin-like, subunit E
Insulin-like growth factor-binding protein 1 Chains: G, H
Molecule details ›
Chains: G, H
Length: 94 amino acids
Theoretical weight: 10.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08833 (Residues: 166-259; Coverage: 40%)
Gene names: IBP1, IGFBP1
Sequence domains: Thyroglobulin type-1 repeat
Structure domains: Invariant Chain; Chain I
Insulin-like growth factor-binding protein 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 92 amino acids
Theoretical weight: 9.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22692 (Residues: 22-113; Coverage: 39%)
Gene names: IBP4, IGFBP4
Sequence domains: Insulin-like growth factor binding protein

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 71.28Å b: 43.66Å c: 81.15Å
α: 90° β: 91.67° γ: 90°
R-values:
R R work R free
0.292 0.288 0.357
Expression system: Escherichia coli BL21(DE3)