PDBe 2drm

X-ray diffraction
1.35Å resolution

Acanthamoeba myosin I SH3 domain bound to Acan125

Entry authors: Houdusse A, Bahloul A, Ostap EM

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Myosin heavy chain IB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 58 amino acids
Theoretical weight: 6.5 KDa
Source organism: Acanthamoeba
Expression system: Escherichia coli BL21
  • Canonical: P19706 (Residues: 1094-1147; Coverage: 5%)
Gene names: MIB, MIL
Sequence domains: SH3 domain
Structure domains: SH3 Domains
18-mer peptide from Acan125 Chains: E, G
Molecule details ›
Chains: E, G
Length: 18 amino acids
Theoretical weight: 1.84 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P1
Unit cell:
a: 29.969Å b: 37.861Å c: 44.506Å
α: 90.28° β: 90.11° γ: 90.64°
R R work R free
0.19 0.186 0.206
Expression systems:
  • Escherichia coli BL21
  • Not provided