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Acanthamoeba myosin I SH3 domain bound to Acan125

X-ray diffraction
1.42Å resolution
PDB entry 2drk coloured by chain, front view.
PDB entry 2drk coloured by chain, side view.
PDB entry 2drk coloured by chain, top view.
Source organism: Acanthamoeba castellanii
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Myosin-I binding protein Acan125 and Myosin heavy chain IB
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Multimeric state: hetero dimer
Accessible surface area: 4365.38 Å2
Buried surface area: 1390.77 Å2
Dissociation area: 522.97 Å2
Dissociation energy (ΔGdiss): 0.21 kcal/mol
Dissociation entropy (TΔSdiss): 7.27 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148693
Assembly 2
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Assembly Name: Myosin-I binding protein Acan125 and Myosin heavy chain IB
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Multimeric state: hetero dimer
Accessible surface area: 4432.45 Å2
Buried surface area: 1326.29 Å2
Dissociation area: 476.48 Å2
Dissociation energy (ΔGdiss): 10.27 kcal/mol
Dissociation entropy (TΔSdiss): 7.09 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148693

Macromolecules

Myosin heavy chain IB
Chain: A
Length: 59 amino acids
Theoretical weight: 6.58 KDa
Source organism: Acanthamoeba castellanii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P19706 (Residues: 1094-1147; Coverage: 5%)
Gene names: MIB, MIL
Pfam: SH3 domain
InterPro:
CATH: SH3 Domains
Myosin heavy chain IB in PDB entry 2drk, assembly 1, front view.
Myosin heavy chain IB in PDB entry 2drk, assembly 1, side view.
Myosin heavy chain IB in PDB entry 2drk, assembly 1, top view.
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Myosin-I binding protein Acan125
Chain: B
Length: 10 amino acids
Theoretical weight: 1.1 KDa
Source organism: Acanthamoeba castellanii
Expression system: Not provided
UniProt:
  • Canonical: P90630 (Residues: 977-986; Coverage: 1%)
Myosin-I binding protein Acan125 in PDB entry 2drk, assembly 1, front view.
Myosin-I binding protein Acan125 in PDB entry 2drk, assembly 1, side view.
Myosin-I binding protein Acan125 in PDB entry 2drk, assembly 1, top view.
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