PDBe 2drk

X-ray diffraction
1.42Å resolution

Acanthamoeba myosin I SH3 domain bound to Acan125

Source organism: Acanthamoeba castellanii
Entry authors: Houdusse A, Bahloul A, Ostap EM

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Myosin heavy chain IB Chain: A
Molecule details ›
Chain: A
Length: 59 amino acids
Theoretical weight: 6.58 KDa
Source organism: Acanthamoeba castellanii
Expression system: Escherichia coli BL21
  • Canonical: P19706 (Residues: 1094-1147; Coverage: 5%)
Gene names: MIB, MIL
Sequence domains: SH3 domain
Structure domains: SH3 Domains
Myosin-I binding protein Acan125 Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.1 KDa
Source organism: Acanthamoeba castellanii
Expression system: Not provided
  • Canonical: P90630 (Residues: 977-986; Coverage: 1%)

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 32.701Å b: 37.362Å c: 43.836Å
α: 90° β: 90° γ: 90°
R R work R free
0.159 0.159 0.173
Expression systems:
  • Escherichia coli BL21
  • Not provided