› 2dor

X-ray diffraction
2Å resolution

DIHYDROOROTATE DEHYDROGENASE A FROM LACTOCOCCUS LACTIS COMPLEXED WITH OROTATE

Released:
DOI: 10.2210/pdb2dor/pdb
PDB entry 2dor coloured by chain, front view.
PDB entry 2dor coloured by chain, side view.
PDB entry 2dor coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
The crystal structure of Lactococcus lactis dihydroorotate dehydrogenase A complexed with the enzyme reaction product throws light on its enzymatic function.
Rowland P, Björnberg O, Nielsen FS, Jensen KF, Larsen S
Protein Sci 7 1269-79 (1998)
PMID: 9655329

Function and Biology Details

Reaction catalysed: 
(S)-dihydroorotate + fumarate = orotate + succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106948 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotate dehydrogenase A (fumarate) Chains: A, B
Molecule details ›
Chains: A, B
Length: 311 amino acids
Theoretical weight: 34.24 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: A2RJT9 (Residues: 1-311; Coverage: 100%)
Gene names: llmg_0952, pyrDA
Sequence domains: Dihydroorotate dehydrogenase
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
1 bound ligand:
Ligand ORO 2 x ORO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 53.91Å b: 109.03Å c: 67.07Å
α: 90° β: 104.43° γ: 90°
R-values:
R R work R free
0.161 0.161 0.187
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Nucleotide metabolism and its control in lactic acid bacteria.
Kilstrup et al. (2005)
2 more

5 mentions without citation

Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies.
Olenginski et al. (2021)
4 more