PDBe 2dga

X-ray diffraction
1.8Å resolution

Crystal structure of hexameric beta-glucosidase in wheat

Released:

Function and Biology Details

Reactions catalysed:
(1) (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H(2)O = 2,4-dihydroxy-7-methoxy-2H-1,4- benzoxazin-3(4H)-one + D-glucose. (2) (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D- glucopyranoside + H(2)O = 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose. 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose. 
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1b, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 565 amino acids
Theoretical weight: 64.17 KDa
Source organism: Triticum aestivum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1XH05 (Residues: 50-569; Coverage: 91%)
Gene name: GLU1B
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P4132
Unit cell:
a: 194.65Å b: 194.65Å c: 194.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.202
Expression system: Escherichia coli