PDBe 2dfk

X-ray diffraction
2.15Å resolution

Crystal structure of the CDC42-Collybistin II complex

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho guanine nucleotide exchange factor 9 Chains: A, C
Molecule details ›
Chains: A, C
Length: 402 amino acids
Theoretical weight: 48.25 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9QX73 (Residues: 71-463; Coverage: 80%)
  • Best match: Q9QX73-2 (Residues: 10-411)
Gene name: Arhgef9
Sequence domains:
Structure domains:
Cell division control protein 42 homolog Chains: B, D
Molecule details ›
Chains: B, D
Length: 194 amino acids
Theoretical weight: 21.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60953 (Residues: 1-191; Coverage: 100%)
Gene name: CDC42
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P212121
Unit cell:
a: 57.957Å b: 147.499Å c: 167.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.229
Expression system: Escherichia coli BL21(DE3)