› 2dd7

X-ray diffraction
1.9Å resolution

A GFP-like protein from marine copepod, Chiridius poppei

Released:
DOI: 10.2210/pdb2dd7/pdb
PDB entry 2dd7 coloured by chain, front view.
PDB entry 2dd7 coloured by chain, side view.
PDB entry 2dd7 coloured by chain, top view.
Source organism: Chiridius poppei
Primary publication:
Structural basis for red-shifted emission of a GFP-like protein from the marine copepod Chiridius poppei.
Suto K, Masuda H, Takenaka Y, Tsuji FI, Mizuno H
Genes Cells 14 727-37 (2009)
PMID: 19469881

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173729 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 24.62 KDa
Source organism: Chiridius poppei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2MHN7 (Residues: 2-219; Coverage: 100%)
Gene name: cpGFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand CXS 1 x CXS
1 modified residue:
Ligand CR2 2 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: C2221
Unit cell:
a: 113.478Å b: 133.494Å c: 108.733Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.232
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

The lineshape of the electronic spectrum of the green fluorescent protein chromophore, part I: gas phase.
Davari et al. (2014)
3 more

2 mentions without citation

Structural Consequences of Chromophore Formation and Exploration of Conserved Lid Residues amongst Naturally Occurring Fluorescent Proteins.
Zimmer et al. (2014)
1 more