PDBe 2dd5

X-ray diffraction
2Å resolution

Thiocyanate hydrolase (SCNase) from Thiobacillus thioparus native holo-enzyme

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thiocyanate hydrolase subunit beta Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 157 amino acids
Theoretical weight: 18.07 KDa
Source organism: Thiobacillus thioparus
UniProt:
  • Canonical: O66186 (Residues: 1-157; Coverage: 100%)
Gene name: scnB
Sequence domains: Nitrile hydratase beta subunit
Thiocyanate hydrolase subunit alpha Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 126 amino acids
Theoretical weight: 14.51 KDa
Source organism: Thiobacillus thioparus
UniProt:
  • Canonical: O66187 (Residues: 1-126; Coverage: 100%)
Gene name: scnA
Sequence domains: Nitrile hydratase beta subunit
Structure domains: SH3 type barrels.
Thiocyanate hydrolase subunit gamma Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 243 amino acids
Theoretical weight: 27.62 KDa
Source organism: Thiobacillus thioparus
UniProt:
  • Canonical: O66188 (Residues: 1-243; Coverage: 100%)
Gene name: scnC
Sequence domains: Nitrile hydratase, alpha chain
Structure domains: Nitrile Hydratase; Chain A

Ligands and Environments

2 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P43212
Unit cell:
a: 170.187Å b: 170.187Å c: 282.469Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.205