2dcr Experiments and Validation

Solution NMR

Entry percentile scores
Source organism: Homo sapiens
Refinement method: Fully automated NMR spectrum analysis and structure calculation without human intervention. Chemical shift assignments and conformational restraints have not been verified manually.
Number of deposited models: 20 models

Sample information

Author description:
Fully automated solution structure determination of the Fes SH2 domain
Source organism: Homo sapiens
Expression system: Cell free synthesis

Validation information

 
Structure ensemble information
Refinement method: Fully automated NMR spectrum analysis and structure calculation without human intervention. Chemical shift assignments and conformational restraints have not been verified manually.
Number of calculated models: 100
Number of deposited models: 20 (structures with the least restraint violations)
Representative model: 1 (medoid: most similar to other models)
 
Experimental NMR data

Experimental information

Sample 1
Contents: 1.2MM UNIFORMLY 13C AND 15N LABELED PROTEIN; 20MM TRIS-HCL BUFFER; 100MM NACL; 1MM DITHIOTHREITOL; 0.02% NAN3 Recorded spectra: 3D_13C-separated_NOESY, 3D_15N-separated_NOESY

Samples and spectra
NMR spectrometers used
Bruker DRX 600 MHz, Bruker Avance 800 MHz