PDBe 2dbt

X-ray diffraction
3.14Å resolution

Crystal structure of chitinase C from Streptomyces griseus HUT6037

Released:

Function and Biology Details

Reaction catalysed:
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta- linkages in chitin and chitodextrins. 

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitinase C Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 265 amino acids
Theoretical weight: 28.5 KDa
Source organism: Streptomyces griseus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O50152 (Residues: 30-294; Coverage: 100%)
Gene name: chiC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P3221
Unit cell:
a: 153.116Å b: 153.116Å c: 90.031Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.174 0.225
Expression system: Escherichia coli BL21(DE3)