PDBe 2d6f

X-ray diffraction
3.15Å resolution

Crystal structure of Glu-tRNA(Gln) amidotransferase in the complex with tRNA(Gln)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Glutamyl-tRNA(Gln) amidotransferase subunit D Chains: A, B
Molecule details ›
Chains: A, B
Length: 435 amino acids
Theoretical weight: 48.05 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O26802 (Residues: 1-435; Coverage: 100%)
Gene names: MTH_706, gatD
Sequence domains:
Structure domains:
Glutamyl-tRNA(Gln) amidotransferase subunit E Chains: C, D
Molecule details ›
Chains: C, D
Length: 619 amino acids
Theoretical weight: 69.63 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O26803 (Residues: 1-619; Coverage: 100%)
Gene names: MTH_707, gatE
Sequence domains:
Structure domains:
tRNA Chains: E, F
Molecule details ›
Chains: E, F
Length: 74 nucleotides
Theoretical weight: 23.84 KDa
Sequence domains: tRNA

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 118.812Å b: 140.706Å c: 186.044Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.292
Expression system: Escherichia coli