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Structural basis for the specificity of basic winged bean lectin for the Tn-antigen: a crystallographic, thermodynamic and modelling study.

Kulkarni KA, Sinha S, Katiyar S, Surolia A, Vijayan M, Suguna K
FEBS Lett 579 6775-80 (2005)
Related entries: 1f9k, 1fay, 1wbf, 1wbl

Cited: 18 times
EuropePMC logo PMID: 16310781

Abstract

The crystal structure of winged bean basic agglutinin in complex with GalNAc-alpha-O-Ser (Tn-antigen) has been elucidated at 2.35 angstroms resolution in order to characterize the mode of binding of Tn-antigen with the lectin. The Gal moiety occupies the primary binding site and makes interactions similar to those found in other Gal/GalNAc specific legume lectins. The nitrogen and oxygen atoms of the acetamido group of the sugar make two hydrogen bonds with the protein atoms whereas its methyl group is stabilized by hydrophobic interactions. A water bridge formed between the terminal oxygen atoms of the serine residue of the Tn-antigen and the side chain oxygen atom of Asn128 of the lectin increase the affinity of the lectin for Tn-antigen compared to that for GalNAc. A comparison with the available structures reveals that while the interactions of the glyconic part of the antigen are conserved, the mode of stabilization of the serine residue differs and depends on the nature of the protein residues in its vicinity. The structure provides a qualitative explanation for the thermodynamic parameters of the complexation of the lectin with Tn-antigen. Modeling studies indicate the possibility of an additional hydrogen bond with the lectin when the antigen is part of a glycoprotein.

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  2. Structure of SO2946 orphan from Shewanella oneidensis shows "jelly-roll" fold with carbohydrate-binding module. Nocek B, Bigelow L, Abdullah J, Joachimiak A. J Struct Funct Genomics 9 1-6 (2008)


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  1. The Tn antigen-structural simplicity and biological complexity. Ju T, Otto VI, Cummings RD. Angew Chem Int Ed Engl 50 1770-1791 (2011)
  2. Plant Lectins Targeting O-Glycans at the Cell Surface as Tools for Cancer Diagnosis, Prognosis and Therapy. Poiroux G, Barre A, van Damme EJM, Benoist H, Rougé P. Int J Mol Sci 18 E1232 (2017)
  3. Deciphering Protein O-GalNAcylation: Method Development and Disease Implication. Yue S, Wang X, Ge W, Li J, Yang C, Zhou Z, Zhang P, Yang X, Xiao W, Yang S. ACS Omega 8 19223-19236 (2023)
  4. Molecular Recognition of GalNAc in Mucin-Type O-Glycosylation. Sanz-Martinez I, Pereira S, Merino P, Corzana F, Hurtado-Guerrero R. Acc Chem Res 56 548-560 (2023)

Articles citing this publication (12)

  1. Lectin microarray profiling of metastatic breast cancers. Fry SA, Afrough B, Lomax-Browne HJ, Timms JF, Velentzis LS, Leathem AJ. Glycobiology 21 1060-1070 (2011)
  2. Structural basis for sugar recognition, including the Tn carcinoma antigen, by the lectin SNA-II from Sambucus nigra. Maveyraud L, Niwa H, Guillet V, Svergun DI, Konarev PV, Palmer RA, Peumans WJ, Rougé P, Van Damme EJ, Reynolds CD, Mourey L. Proteins 75 89-103 (2009)
  3. Comparative study of protein-protein interaction observed in PolyGalacturonase-Inhibiting Proteins from Phaseolus vulgaris and Glycine max and PolyGalacturonase from Fusarium moniliforme. Maulik A, Ghosh H, Basu S. BMC Genomics 10 Suppl 3 S19 (2009)
  4. High-resolution structure of a new Tn antigen-binding lectin from Vatairea macrocarpa and a comparative analysis of Tn-binding legume lectins. Sousa BL, Silva Filho JC, Kumar P, Pereira RI, Łyskowski A, Rocha BA, Delatorre P, Bezerra GA, Nagano CS, Gruber K, Cavada BS. Int J Biochem Cell Biol 59 103-110 (2015)
  5. Engineering O-glycosylation points in non-extended peptides: implications for the molecular recognition of short tumor-associated glycopeptides. Corzana F, Busto JH, Marcelo F, García de Luis M, Asensio JL, Martín-Santamaría S, Jiménez-Barbero J, Avenoza A, Peregrina JM. Chemistry 17 3105-3110 (2011)
  6. Generation of blood group specificity: new insights from structural studies on the complexes of A- and B-reactive saccharides with basic winged bean agglutinin. Kulkarni KA, Katiyar S, Surolia A, Vijayan M, Suguna K. Proteins 68 762-769 (2007)
  7. A Distribution-Free Convolution Model for background correction of oligonucleotide microarray data. Chen Z, McGee M, Liu Q, Kong M, Deng Y, Scheuermann RH. BMC Genomics 10 Suppl 1 S19 (2009)
  8. Studies on the interactions between glycosylated beta3-peptides and the lectin Vicia villosa by saturation transfer difference NMR spectroscopy. Kaszowska M, Norgren AS, Arvidson PI, Sandström C. Carbohydr Res 344 2577-2580 (2009)
  9. Crystal structure of the legume lectin-like domain of an ERGIC-53-like protein from Entamoeba histolytica. Khan F, Suguna K. Acta Crystallogr F Struct Biol Commun 75 197-204 (2019)
  10. Molecular dynamics and binding energy analysis of Vatairea guianensis lectin: a new tool for cancer studies. Cavada BS, Osterne VJS, Pinto-Junior VR, Souza LAG, Lossio CF, Silva MTL, Correia-Neto C, Oliveira MV, Correia JLA, Neco AHB, Domingos JLC, Ferreira WP, Farias GA, Nascimento KS. J Mol Model 26 22 (2020)
  11. Remarkable Homeostasis of Protein Sialylation in Skeletal Muscles of Hibernating Daurian Ground Squirrels (Spermophilus dauricus). Dang K, Yu HJ, Xu SH, Ma TR, Wang HP, Li Y, Li Z, Gao YF. Front Physiol 11 37 (2020)
  12. cDNA cloning, molecular modeling and docking calculations of L-type lectins from Swartzia simplex var. grandiflora (Leguminosae, Papilionoideae), a member of the tribe Swartzieae. Maranhão PAC, Teixeira CS, Sousa BL, Barroso-Neto IL, Monteiro-Júnior JE, Fernandes AV, Ramos MV, Vasconcelos IM, Gonçalves JFC, Rocha BAM, Freire VN, Grangeiro TB. Phytochemistry 139 60-71 (2017)


Related citations provided by authors (3)

  1. Carbohydrate specificity and quaternary association in basic winged bean lectin: X-ray analysis of the lectin at 2.5 A resolution.. Prabu MM, Sankaranarayanan R, Puri KD, Sharma V, Surolia A, Vijayan M, Suguna K J Mol Biol 276 787-96 (1998)
  2. Structure of basic winged-bean lectin and a comparison with its saccharide-bound form.. Manoj N, Srinivas VR, Suguna K Acta Crystallogr D Biol Crystallogr 55 794-800 (1999)
  3. Carbohydrate specificity and salt-bridge mediated conformational change in acidic winged bean agglutinin.. Manoj N, Srinivas VR, Surolia A, Vijayan M, Suguna K J Mol Biol 302 1129-37 (2000)

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